Galectin 3 anticorps (AA 139-250)

N° du produit ABIN6719486

L’anticorps Souris Monoclonal anti-Galectin 3 a été validé pour WB, IHC, IF, FACS et ICC. Il convient pour détecter Galectin 3 dans des échantillons de Humain, Souris et Rat.

Aperçu rapide pour Galectin 3 anticorps (AA 139-250) (ABIN6719486)

Antigène: Galectin 3 (LGALS3)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Galectin 3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 12B12

Détail du produit anti-Galectin 3 anticorps

Épitope: AA 139-250

Fonction: Anti-Galectin 3/LGALS3 Antibody Picoband® (monoclonal, 12B12)

Réactivité croisée (Details): No cross-reactivity with other proteins.

Attributs du produit: Anti-Galectin 3/LGALS3 Antibody Picoband® (monoclonal, 12B12) (ABIN6719486). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Purification: Immunogen affinity purified.

Immunogène: E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequence identity with mouse and rat Galectin 3, respectively.

Isotype: IgG2a

Information d'application

Indications d'application: Western blot, 0.1-0.5 μg/mL
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL
Immunocytochemistry/Immunofluorescence, 2 μg/mL
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells
1. Dumic J, Dabelic S, Flögel M. (2006). "Galectin-3: an open-ended story.". BBA General Subjects 1760(4): 616-635. 2. Liu F, Patterson RJ, Wang JL. (2002). "Intracellular function of galectins.". BBA General Subjects 1572 (2-3): 263-273.  3. Cooper D. (2002). "Galectinomics: finding themes in complexity.". BBA General Subjects 1572 (2-3): 209-231. 4. Raimond J, Zimonjic DB, Mignon C, et al. (1997). "Mapping of the galectin-3 gene (LGALS3) to human chromosome 14 at region 14q21-22.". Mamm. Genome 8 (9): 706-707.

Commentaires:

Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users.

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Concentration: 500 μg/mL

Buffer: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

Détails sur Galectin 3

Antigène: Galectin 3 (LGALS3)

Autre désignation: LGALS3

Sujet:

Synonyms: Galectin-3, Gal-3, 35 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, Mac-2 antigen, LGALS3, MAC2

Tissue Specificity: A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes.

Background: SAM domain and HD domain-containing protein 1 is a protein that in humans is encoded by the SAMHD1 gene. This gene may play a role in regulation of the innate immune response. The encoded protein is upregulated in response to viral infection and may be involved in mediation of tumor necrosis factor-alpha proinflammatory responses. Mutations in this gene have been associated with Aicardi-Goutieres syndrome.

Poids moléculaire: 28 kDa

ID gène: 3958

UniProt: P17931

Pathways: Signalisation RTK